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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: A circular RNA derived from PLXNB2 as a valuable predictor of the prognosis of patients with acute myeloid leukaemia

Fig. 2

Expression of circPLXNB2 and PLXNB2 mRNA, and characterization and distribution of circPLXNB2 in AML cells. The expression of circPLXNB2 (a) and the PLXNB2 mRNA (b) in different AML cell lines was detected using qRT-PCR; BMMCs from healthy volunteers served as controls. qRT-PCR analysis was used to verify that transfection of OCI-AML3 cells (c) and HL-60 cells (d) with circPLXNB2 shRNA or shRNA NC or vector or circPLXNB2 OE plasmid was successful. e AML cells were treated with or without RNase R, and qRT-PCR was used to assess circPLXNB2 amplification with divergent primers and convergent primers using the template cDNA and gDNA derived from AML cells. f qRT-PCR assay of the expression of circPLXNB2 and the PLXNB2 mRNA in AML cells treated with the transcriptional inhibitor actinomycin D (2 μg/mL) at the indicated time points. The PLXNB2 mRNA was used as a control. g Identification of the circPLXNB2 distribution in OCI-AML3 cells and HL-60 circPLXNB2 OE cells using RNA-FISH. h Quantification of nuclear and cytoplasmic levels of circPLXNB2 staining in AML cells. Nuclei were stained with DAPI. The original magnification was 400×. Each experiment was repeated three times. *P < 0.05, **P < 0.01, and ***P < 0.001. CircPLXNB2 OE circPLXNB2 overexpression, circPLXNB2 shRNA circPLXNB2 short hairpin RNA, NC negative control, DAPI 4,6-diamidino-2-phenylindole, cDNA complementary DNA, gDNA genomic DNA, ns no significance

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