Fig. 2

CMTM6 promotes proliferation, migration, and invasion of HCC cells. a Western blotting analysis of CMTM6 levels in HCC cell lines. b Western blotting analysis of CMTM6 levels in MHCC-97H and BEL-7402 cells. MHCC-97H and BEL-7402 cells were transduced with a lentivirus encoding a CMTM6 shRNA or scrambled shRNA. c Cell proliferation was determined using the CCK-8 assay in HCC cells expressing the CMTM6 shRNA. d Colony-forming efficiency of HCC cells. The cells were transduced with lentivirus encoding the CMTM6 shRNA or scrambled shRNA. e The cellular migration abilities were examined using a wound healing assay in HCC cells expressing the CMTM6 shRNA. f Cell invasion was evaluated using Transwell assays in in HCC cells expressing the CMTM6 shRNA. g Western blotting analysis of CMTM6 expression in HCCLM3 cells transduced with lentivirus expressing CMTM6 or with the empty vector. h Cell proliferation was determined using the CCK-8 assay in HCCLM3 overexpressing CMTM6. i Colony-forming efficiency of HCCLM3 cells overexpressing CMTM6. j Cell invasion was examined using Transwell assays in HCCLM3 cells overexpressing CMTM6. k Wound healing assay