Fig. 4

Characterization of small RNAs (18–32 bp) identified by RNA-seq. a Heatmap representing the consistency of the profiles of small RNAs obtained via the four EVs isolation methods. b Percentage of miRNA reads identified by database miRbase (V22) and software miRDeep2 (exoEasy: 8.14%, ExoQuick: 22.75%, SEC: 32.98%, UC: 22.09%). c Percentage of mapped reads based on the reference genome (exoEasy: 29.5%, ExoQuick: 55.61%, SEC: 61.11%, UC: 45.82%)