Skip to main content
Fig. 6 | Journal of Translational Medicine

Fig. 6

From: mTOR regulates GPVI-mediated platelet activation

Fig. 6

The PKCδ inhibitor rottlerin restores the aggregation and dense granule secretion (ATP release) of mTOR−/− platelets in response to low-dose collagen. ac Wash platelets were pre-incubated with DMSO (vehicle), rottlerin (5 μM) or df Go6976 (40 nM) for 10 min and then stimulated with collagen at the indicated low dose (0.5 μg/mL). The PKCδ inhibitor rottlerin, but not the PKCα/β inhibitor Go6976, restored the full aggregation of mTOR−/− platelets in response to low-dose collagen (0.5 μg/mL). Data from at least three independent experiments were quantified and expressed as means ± SEM (*P < 0.05, **P < 0.01, Paired Student’s t test). The arrows show “aggregation” or “ATP Release,” indicating 90% light transmission or 70% ATP release, respectively

Back to article page