Fig. 3

mTOR^−/− platelets exhibit impaired activation of α_IIbβ₃ after induction with low concentrations of collagen/CRP or the PAR agonist thrombin. Washed platelets were pre-incubated with phycoerythrin-labeled rat anti–mouse integrin α_IIbβ₃ mAb (JON/A). Then, they were activated with thrombin, collagen/CRP. The mean fluorescence intensity (MFI) was measured by flow cytometry. Results are expressed as MFI ± SEM (n ≥ 3). The data were analyzed for statistical significance using the Student’s t test (*P < 0.05, **P < 0.01)