Fig. 2

mTOR^−/− platelets exhibit impaired aggregation and dense granule secretion (ATP release) after stimulation of GPVI with low-dose CRP or stimulation with the PAR agonist GYPGKF-NH₂. a Washed platelets from mTOR^−/− mice and WT littermates were challenged with CRP (0.75 μg/mL or 4 μg/mL) or b the PAR4 agonist GYPGKF-NH₂ (0.75 mM or 3.2 mM), and their aggregation and simultaneous ATP release were measured; c PRP was stimulated with ADP (4 μM or 30 μM) and platelet aggregation were measured; d–j The calculated percentages of aggregation and ATP release. Results are presented as means ± SEM from at least three experiments (*P < 0.05, **P < 0.01, paired Student’s t test). The arrows show “aggregation” or “ATP Release,” indicating 90% light transmission or 70% ATP release, respectively