Fig. 2

Midkine and ACE level in plasma and lung tissue. a Plasma midkine in CLP (n = 6) vs. sham (n = 6) model [ng/L, 747.3 (703.6–811.1) vs. 87 (64.5–199.5), P = 0.0095]. b Plasma ACE in CLP (n = 6) vs. sham (n = 6) [ng/mL, 454.1 (378.1–500.3) vs. 53.4 (46.3–63.8), P = 0.0022]. c Plasma angiotensin II in CLP (n = 6) vs. sham (n = 6) [ng/L, 7.2 (6.8–9.6) vs. 1.9 (1.2–3.3), P = 0.002]. d Midkine and ACE mRNA level in lung tissue in CLP (n = 6) vs. sham (n = 6), mRNA amplification was normalized to β-actin. e Midkine and ACE protein level was determined by Western blots, and the representative result was shown. Quantitative analysis of midkine and ACE protein using densitometry, normalized to β-actin