Fig. 2

Gene Ontology enrichment analysis of differentially expressed genes (DEGs) between FOXL2^C134W and FOXL2^WT. a Volcano plot showing in pink DEGs between FOXL2^C134W and FOXL2^WT (DESeq, q < 0.05). The genes FOXL2 and SMAD3 were omitted from the plot. b, c Gene ontology enrichment analysis using up-regulated genes in FOXL2^C134W transfected cells (b, n = 487), and down-regulated genes in FOXL2^C134W transfected cells (C, n = 230). The red line indicates p-value cutoff for p < 0.05. Gene ontology enrichment p-values were calculated using Metascape [51]. GO terms associated with expression of FOXL2 and/or SMAD3 were removed from the results (see Methods and Additional file 2: Tables S2–4)