Fig. 2
Phenotypic analysis of the meso3 CAR-T and meso3-CD40 CAR-T cell products. a The percentages of TCM and TEM in meso3 CAR-T and meso3-CD40 CAR-T cells were detected by flow cytometry. Memory T cells were gated using an anti-human CD197 antibody and an anti-human CD62L antibody from CD45RO+ T cells. b The summarized statistics regarding the memory T cells from three donors are shown in the bar graphs (n = 3, mean ± SEM, ns, not significant, *P < 0.05; **P < 0.01; ***P < 0.001). c–e Activation markers on the surface of T cells were detected by flow cytometry upon stimulation with the rhMSLN antigen for 24 h. f The summarized statistics of the activation markers on the CAR-T cells from three donors are shown in the bar graphs (n = 3, mean ± SEM, ns, not significant)