Skip to main content
Fig. 1 | Journal of Translational Medicine

Fig. 1

From: Chimeric antigen receptor T cells engineered to secrete CD40 agonist antibodies enhance antitumor efficacy

Fig. 1

Construction and characterization of meso3 CAR-T and meso3-CD40 CAR-T cells. a Schematic representation of the plasmid vector encoding the anti-CD40 antibody and meso3 CAR. b Ten days after electroporation, the positive ratio of meso3 CAR in meso3 CAR-T and meso3-CD40 CAR-T cells was detected by flow cytometry. c The summarized statistics of three donors are shown in the bar graphs (n = 3, mean ± SEM, ns, not significant). d The anti-CD40 antibody levels in the supernatants of meso3 CAR-T and meso3-CD40 CAR-T cells were determined by ELISA, and the results are shown in the bar graphs (n = 3, mean ± SEM). e, f CD40 expression in meso3 CAR-T and meso3-CD40 CAR-T cells after stimulation with rhMSLN for different amounts of times

Back to article page