Fig. 5

Ferroptosis mediates inflammation in LPS-treated BEAS-2B cells. The cells are treated with ferroptotic inhibitor Fer-1 (0.1 μM) or DFO (10 μM), or ferroptotic inducer Fe (5 M) or RSL3 (0.2 μM) in the presence of LPS for 24 h. Then ferroptosis and inflammation are assessed. a Cell viability determined by MTT assay. b Cell death detected by FDA staining kit. c Fe²⁺ level determined using Iron Assay Kit. d MDA level detected via the MDA Assay Kit. e GSH level detected by GSH assay kit. f GPX4 activity measured by GPX4 ELISA kit. g GPX4 mRNA expression decided by RT-PCR. h GPX4 protein expression detected by western blot assay. i TNF-α, IL-1β, and IL-6 level in cell culture supernatants determined via ELISA Kits. ^#P <0.05 vs. LPS group