Fig. 2
Ferroptosis is increased in LPS-induced acute lung injury. The ferroptosis level is evaluated in lung tissues via detection of the biomarkers of ferroptosis. a Fe2+ level determined using Iron Assay Kit. b Malondialdehyde (MDA) level detected via the MDA Assay Kit. c Glutathione (GSH) level detected by GSH assay kit. d Glutathione Peroxidase 4 (GPX4) activity measured by GPX4 ELISA kit. e GPX4 mRNA expression determined by RT-PCR. f GPX4 protein expression detected by western blot assay. *P < 0.05 vs. ctrl group