Fig. 1

Successful establishment of LPS-induced acute lung injury in vivo. The mice are anesthetized with 30 mg/kg of pentobarbital sodium, and then LPS (10 µg/mouse) or stroke-physiological saline solution (SPSS) is injected into the trachea. Then bronchoalveolar lavage fluid (BALF) and lung tissue are collected used as the following detections. a The lung histological changes are assessed by haematoxylin and eosin (H&E) staining. b The lung injury scores are assessed by a blinded pathologist. c The lung Wet/Dry (W/D) weight ratio. d Myeloperoxidase (MPO) activity in lung tissues detected by a MPO Detection Kit. e Pro-inflammatory cytokines TNF-α, IL-1β, and IL-6 level in BALF determined by ELISA kits. *P < 0.05 vs. ctrl group