Table 1 Comparison of the rRT-PCR assay and DETECTR (RT–LAMP/Cas12) assay with the OR-DETECTR assay for detection of SARS-CoV-2
From: One-tube SARS-CoV-2 detection platform based on RT-RPA and CRISPR/Cas12a
rRT-PCR | DETECTR [13] | OR-DETECTR | |
|---|---|---|---|
LoD (sensitivity) | 1 copy/μl input 3.2 copies/μl input [19] | 10 copies/μl input | 2.5 copies/μl input (RNA standard) 1 copy/μl input (Pseudovirus) |
Cross-react (specificity) | No | No | No |
Amplification method | PCR | LAMP | RPA |
Difficulty of primer design | Easy | Difficult | Easy |
Turnaround time | 120 min | 40 min | 50 min |
One or more tubes | One | More | One |
Temperature | Constant change | 62–37 °C | 42 °C |
Bulky instrumentation required | Yes | Yes/No | Yes/No |
Detection of target | N1/N2 | E/N | RdRp/N |
Results | Quantitative | Qualitative | Qualitative |
Cost (each person) | Around ¥20 | Around ¥20 | Around ¥20 |