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Table 1 Comparison of the rRT-PCR assay and DETECTR (RT–LAMP/Cas12) assay with the OR-DETECTR assay for detection of SARS-CoV-2

From: One-tube SARS-CoV-2 detection platform based on RT-RPA and CRISPR/Cas12a

  rRT-PCR DETECTR [13] OR-DETECTR
LoD (sensitivity) 1 copy/μl input
3.2 copies/μl input [19]
10 copies/μl input 2.5 copies/μl input (RNA standard)
1 copy/μl input (Pseudovirus)
Cross-react (specificity) No No No
Amplification method PCR LAMP RPA
Difficulty of primer design Easy Difficult Easy
Turnaround time 120 min 40 min 50 min
One or more tubes One More One
Temperature Constant change 62–37 °C 42 °C
Bulky instrumentation required Yes Yes/No Yes/No
Detection of target N1/N2 E/N RdRp/N
Results Quantitative Qualitative Qualitative
Cost (each person) Around ¥20 Around ¥20 Around ¥20