Fig. 2

OR-DETECTR assay for SARS-CoV-2 in standard RNA and clinical oropharyngeal swab samples. a Fold change data (OR-DETECTR) on RdRp gene and CT values (rRT-PCR) on Orf1ab of patient samples. There were 14 RNA samples from 14 suspected COVID-19 patients. N50S was one RNA mixed sample from 40 fever patients. The CT value of each RNA sample was larger than 40 and undetected. The blue pentagrams (star) represent the CT values of the samples on Orf1ab gene, and the blue pentagrams exceeding the coordinate axis represent the CT values of the samples on Orf1ab gene greater than 40. The black columns represent the FC values of samples on RdRp gene, and the cutoff was 1.6. b Fold change data (OR-DETECTR) and CT values (rRT-PCR) on the N gene of suspected COVID-19 patient samples. The samples were the same as a. The red pentagrams (star) represent the CT values of the samples on the N gene, and the red pentagrams exceeding the coordinate axis represent the CT values of the samples on the N gene greater than 40. The black columns represent the FC values samples on the N gene, and the cutoff of the N gene was 2.6. c, d LoD for OR-DETECTR assay. SARS-CoV-2 RdRp and N gene standard RNA mix were used as the samples for evaluating LoD of OR-DETECTR assay. Standard RNA mix is serially diluted, and there were 7 replicates per dilution. e Visual OR-DETECTR assay by test card with lateral flow strip. Standard RNA mix is serially diluted, and there were 2 replicates per dilution