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Table 4 qPCR-validation of selected genes from RNASeq

From: Insulin treatment improves liver histopathology and decreases expression of inflammatory and fibrogenic genes in a hyperglycemic, dyslipidemic hamster model of NAFLD

Gene symbol NASH-STZ vs NASH NASH-STZ-HI vs NASH-STZ
  RNASeq (log2-fold change) q-value qPCR (log2-fold change) q-value RNASeq (log2-fold change) q-value qPCR (log2-fold change) q-value
Gpnmb 5.43 2.00 × E−03 5.55 2.94 × E−05 − 2.92 2.96 × E−03 − 2.65 1.43 × E−02
Pklr − 3.35 3.10 × E−03 − 3.58 3.22 × E−04 3.25 3.65 × E−03 3.55 3.48 × E−04
Spp1 4.31 2.14 × E−03 4.74 1.01 × E−05 − 3.01 3.77 × E−03 − 3.04 9.20 × E−04
Soat1 3.64 1.02 × E−03 3.62 1.71 × E−06 − 2.84 2.28 × E−03 − 2.50 1.14 × E−04
Ccl2 2.78 4.76 × E−03 3.19 1.25 × E−03 − 1.96 7.58 × E−03 − 1.72 5.10 × E−02
Il1β 1.72 < 0.05 1.54 4.16 × E−03 − 1.21 < 0.05 − 0.82 9.31 × E−02
Col3a1 1.64 1.93 × E−03 1.70 7.17 × E−05 − 0.96 5.06 × E−03 − 0.94 9.69 × E−03
Timp1 2.71 3.20 × E−03 2.69 3.13 × E−04 − 1.69 2.18 × E−03 − 1.57 1.70 × E−02