Fig. 1

Overview of study design. Animals were assigned to receive either a control diet (one group, n = 10) or a NASH-diet (three groups, n = 10/group) for four weeks. After four weeks, two of the NASH-diet-fed groups (NASH-STZ, and NASH-STZ-HI) were injected subcutaneously with STZ (40 mg/kg) on three consecutive days, while the control-diet group (CTRL) and the remaining NASH-diet-group (NASH) were sham-injected with saline. One week after STZ/Sham-injections, osmotic minipumps containing either vehicle (CTRL, NASH, NASH-STZ) or Human Insulin (NASH-STZ-HI) were surgically inserted subcutaneously. After a four-week treatment period the animals were euthanized, and tissue samples were collected. STZ: Streptozotocin