Fig. 6

The roles of RVS in the autophagy activities of RAW264.7 insulted by CQ and PI3K/Akt/mTOR signaling pathway regulating autophagy. a Representative images of western blot showing LC3 and p62 expression in RAW264.7 cells in different groups. b Immunofluorescence staining for LC3II in cells, scale bar = 50 μm. c Representative images of ORO-stained lipid droplets. d Immunblots and densitometry values of western blot analysis of Arg-1 and iNOS. e qRT-PCR mRNA evaluation of genes involved in polarization of macrophages. Effects of f PI3K agonist 740 Y-P and g PI3K inhibitor LY294002 on LC3II/I ratio and p62 level in ox-LDL-stimulated macrophages. Data were expressed as the mean ± SD from three independent experiments. Four randomly selected visual fields were counted in each group. *p < 0.05, **p < 0.01