Fig. 5

RVS regulated lipid deposition and polarization shift of macrophages via mediating autophagy. a Western blot analysis of the levels of autophagy-related molecules. b Immunofluorescence staining of LC3II in RAW264.7 cells after different treatments, scale bar = 50 μm. c Representative ORO staining images of macrophages with different treatment. d ABCA1 and ABCG1 expression levels in different groups. e Immunofluorescence staining for iNOS and Arg-1 was performed, scale bar = 50 μm. Data were expressed as the mean ± SD from three independent experiments. Four randomly selected visual fields were counted in each group. *p < 0.05, **p < 0.01