Fig. 3

Long-term L. edodes derived β-glucan supplementation maintained colonic integrity and alleviated inflammation induced by HF diet. a Representative figure of alcian blue staining for colon, b quantification of colonic mucus layer thickness, c protein expression levels of occludin in the colon, d plasma LPS levels, e the representative image of immunohistochemical staining of colonic F4/80-positive cells, f quantification of colonic F4/80-positive cells, g mRNA expression of TNF-α, IL-6 and IL-1β in the colon. Data are presented as mean ± SEM. n = 3 per group. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, vs lab chow diet group (LC); ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, vs high-fat diet group (HF). HFL, L. edodes β-glucan supplementation in HF diet group