Fig.2

SIK1 was down-regulated in AA-induced AKI-CKD transition mice. a ELISA analysis of IL-1β, and TNF-α expression in different groups of mice. b Western blot and real-time PCR analysis of inflammation and fibrosis markers (Caspase1/p20/p10, FN and COL1) expression; c Western blot and real-time PCR analysis of EMT markers (E-cadherin, Vimentin, and α-SMA) expression. d Representative immunohistochemical staining images of E-cadherin in different groups of mice. Scale bar = 50 μm. The graph showed the quantitative analysis of E-cadherin in immunohistochemically stained sections. e The kidney index, Scr, BUN and 24 h urinary protein levels in different groups of mice. f Representative histological staining (HE, PAS, and Masson's trichrome staining) images of kidney tissues in different groups of mice. Scale bar = 50 μm. g Western blot analysis of SIK1 and p-SIK (Thr182) protein levels in C57BL/6 mice. Data are shown as mean ± s.d. *P < 0.05 vs Control. All experiments were performed in triplicate