Fig. 1

Chromosomal loops structure and conformation assay. a Chromosomal loop contains the enhancer region that increases target gene promoter activity. b During cancer progression there is increased looping in some tumour-related genes leading to abnormal gene expression. c Chromosome conformation assay: DNA is crosslinked, digested, ligated and new sequences (in places where loops were) are predicted using relevance machine vector. Loops presence is then detected using DNA microarray. Resulting markers are analysed using multivariate analysis yielding specific epigenetic signatures for selected patient cohorts