Fig. 6

AZD8931 increases CD8 + T cell content in B4B8 tumors and therapeutic activity is diminished in immune-deficient mice. a B4B8 tumors treated with diluent or AZD8931 (50 mg/kg) for 3 (control, n = 1–2; treated, n = 2), 7 (control, n = 4; treated, n = 4), or 14 (control, n = 2; treated, n = 2) days were formalin fixed, paraffin embedded and submitted to immunofluorescence staining for the T cell marker CD3e with CD4 or CD8α. Five ROIs per tumor were randomly selected (accounting for > 70% of the section area). Two independent observers quantified each field of view for the number of positively stained cells per field and the average of the two independent observations and SD are reported. b Representative immunofluorescence images of CD3e + (green), CD8α + (red) and DAPI stained B4B8 tumors treated with diluent or AZD8931 (50 mg/kg). c B4B8 tumors were implanted in nu/nu mice or syngeneic BALB/c mice and treated with AZD8931 (50 mg/kg). The percent change in tumor volume of individual tumors after 17 days of treatment with AZD8931 in BALB/c and nu/nu mice is presented as a waterfall plot