Fig. 3

ERp44 could interact with ACLY in NPC cells. a The results from string protein interaction network, it showed the proper proteins that might interact with ERp44. b Co-IP was used to confirm the interaction of ERp44 with ACLY. c The mutant variants of ERp44 containing the tag HA, mutations in the thioredoxin region (ERp44-Δ 1, mutant in 30–138), mutations in other than thioredoxin region (ERp44-Δ 2, mutant in 139–402). d Co-IP confirmed that ERp44-Δ1was the interaction region with ACLY in 293 T and CNE2 cells. e Western blot showed that when ERp44 was knockdown, there was similar tread of expression change in ACLY. All experiments were repeated three times. Data represent mean ± SEM. *p < 0.05