Fig. 1
From: Patient-derived organoid (PDO) platforms to facilitate clinical decision making
Schematic representation of four models for tumor cell culture in vitro. 2D cell line: after obtaining cells from ATCC, they can be grown in a 10 cm dish, and then visualized using light microscopy. 3D cell line: after obtaining cells from ATCC, they can be grown in a low attachment 96 well plate. 2D primary cells: after obtaining primary cells from a bladder cancer tumor, the cells can be grown in a 10 cm dish. However, after several passages, they lose their heterogeneity. Organoids: after obtaining primary cells from a bladder cancer tumor, the cells can be plated as organoids in 60 µL Matrigel droplets