Fig. 6
From: Validation of a method evaluating T cell metabolic potential in compliance with ICH Q2 (R1)
Range of the method. a Repeatability assessment of OCR or ECAR detection within the linear area: illustration of mean ± SD of OCR (up) and ECAR (down) levels (6 technical replicates, three times of measurement) at baseline, after oligomycin and after FCCP addition, cells/well concentration of 25,000 (very light grey bar), 50,000 (light grey bar), 100,000 (dark grey bar) and 150,000 (black bar), associated table of statistical metric at baseline, after oligomycin and after FCCP addition. CV: coefficient of variation, expressed in percentage. b Intermediate precision assessment of OCR or ECAR detection within the linear repeatable area: illustration of mean ± SD of OCR (up) and ECAR (down) levels (average values of 3 independent experiments) at baseline, after oligomycin and after FCCP addition, cells/well concentration of 50,000 (light grey bar), 100,000 (dark grey bar) and 150,000 (black bar), associated table of statistical metric at baseline, after oligomycin and after FCCP. CV: coefficient of variation, expressed in percentage