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Table 6 Isolation method characteristics

From: Qualitative and quantitative comparison of cell-free DNA and cell-free fetal DNA isolation by four (semi-)automated extraction methods: impact in two clinical applications: chimerism quantification and noninvasive prenatal diagnosis

Manufacturer Roche ID-Solution Taigen Perkin Elmer
Robot MagNA Pure 24 System IDEAL LabTurbo 24 AB Chemagic 360D
A B C D
Product MagNA Pure 24
Total NA Isolation Kit
IDXTRACT-MAG Virus combo kit 24C-LVX480-1000 NextPrep-Mag cf DNA isolation kit
Type Bead based Bead based Column Bead based
Sample volume (mL) 2–4 0.5–5 0.3–3 0.5–10
Elution volume (µL) 30–200 30–120 (adaptable) 30–200 30–100
Level of automation Full Semi Full Semi
Manual pre processing   Reconstitute the PK and incubation (5 min at room temperature), addition of beads + buffers (incubation with vigorous shaking for 30 min at 56 °C). Adaptable distribution of buffers and elution reagents Wash preparation: addition of EtOH Add beads, add elution reagent in a bar or tube, reconstitute the Proteinase K
Hands-on-time (min) 0 45 5 20
Automated runtime (min) 90 45 70 70
Total runtime (min) 90 90 75 90
Samples 24 24 24 12
Cost of the instrument + + + ++
Cost of consumables ++ + ++ +
Dimensions (cm)
 Height × width × length 77.5 × 76.5 × 89.9 37 × 57 × 72 87 × 74 × 65 90 × 82 × 90