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Table 5 Quantification results of 3 Rhesus exons of the different isolation methods using DNA Fetal Kit® RHD CE (BIORAD®)

From: Qualitative and quantitative comparison of cell-free DNA and cell-free fetal DNA isolation by four (semi-)automated extraction methods: impact in two clinical applications: chimerism quantification and noninvasive prenatal diagnosis

Isolation methods Origin samples Weeks Expected results Results
Ct exon 5 Ct exon 7 Ct exon 10 Ct exonegous DNA Results
MagNA Pure 24 System Sample 1 15 Positive 37.12 35.7 38.02 35.61 Positive
Sample 2 27 Positive 34.24 35.18 35.2 35.61 Positive
Sample 3 16 Negative > 40 > 40 > 40 36.24 Negative
Control D+ ND Positive 33.18 34.02 34.5 35.42 Positive
Control D− ND Negative > 40 > 40 > 40 34.66 Negative
IDEAL Sample 4 24 Negative > 40 > 40 > 40 33.65 Negative
Sample 5 20 Positive 38.1 36.88 36.78 34.37 Positive
Sample 6 14 Positive 36.22 35.15 36.8 33.79 Positive
Control D+ ND Positive 32.09 33.12 33.3 33.42 Positive
Control D− ND Negative > 40 > 40 > 40 33.32 Negative
LabTurbo 24 AB Sample 7 21 Positive 34.89 36 36.12 33.08 Positive
Sample 8 19 Negative > 40 > 40 > 40 32.52 Negative
Sample 9 14 Positive 37.33 37.18 37.82 32.59 Positive
Control D+ ND Positive 32.27 33.08 33.88 33.25 Positive
Control D- ND Negative > 40 > 40 > 40 32.86 Negative
Chemagic 360D Sample 10 18 Positive 37.63 37.45 37.87 37.22 Positive
Sample 11 18 Negative > 40 > 40 > 40 35.64 Negative
Sample 12 21 Positive 36.34 38.22 40 38.39 Positive
Control D+ ND Positive 35.07 35.44 37.07 38.66 Positive
Control D− ND Negative > 40 > 40 > 40 39.38 Negative