Table 5 Quantification results of 3 Rhesus exons of the different isolation methods using DNA Fetal Kit® RHD CE (BIORAD®)
Isolation methods | Origin samples | Weeks | Expected results | Results | ||||
|---|---|---|---|---|---|---|---|---|
Ct exon 5 | Ct exon 7 | Ct exon 10 | Ct exonegous DNA | Results | ||||
MagNA Pure 24 System | Sample 1 | 15 | Positive | 37.12 | 35.7 | 38.02 | 35.61 | Positive |
Sample 2 | 27 | Positive | 34.24 | 35.18 | 35.2 | 35.61 | Positive | |
Sample 3 | 16 | Negative | > 40 | > 40 | > 40 | 36.24 | Negative | |
Control D+ | ND | Positive | 33.18 | 34.02 | 34.5 | 35.42 | Positive | |
Control D− | ND | Negative | > 40 | > 40 | > 40 | 34.66 | Negative | |
IDEAL | Sample 4 | 24 | Negative | > 40 | > 40 | > 40 | 33.65 | Negative |
Sample 5 | 20 | Positive | 38.1 | 36.88 | 36.78 | 34.37 | Positive | |
Sample 6 | 14 | Positive | 36.22 | 35.15 | 36.8 | 33.79 | Positive | |
Control D+ | ND | Positive | 32.09 | 33.12 | 33.3 | 33.42 | Positive | |
Control D− | ND | Negative | > 40 | > 40 | > 40 | 33.32 | Negative | |
LabTurbo 24 AB | Sample 7 | 21 | Positive | 34.89 | 36 | 36.12 | 33.08 | Positive |
Sample 8 | 19 | Negative | > 40 | > 40 | > 40 | 32.52 | Negative | |
Sample 9 | 14 | Positive | 37.33 | 37.18 | 37.82 | 32.59 | Positive | |
Control D+ | ND | Positive | 32.27 | 33.08 | 33.88 | 33.25 | Positive | |
Control D- | ND | Negative | > 40 | > 40 | > 40 | 32.86 | Negative | |
Chemagic 360D | Sample 10 | 18 | Positive | 37.63 | 37.45 | 37.87 | 37.22 | Positive |
Sample 11 | 18 | Negative | > 40 | > 40 | > 40 | 35.64 | Negative | |
Sample 12 | 21 | Positive | 36.34 | 38.22 | 40 | 38.39 | Positive | |
Control D+ | ND | Positive | 35.07 | 35.44 | 37.07 | 38.66 | Positive | |
Control D− | ND | Negative | > 40 | > 40 | > 40 | 39.38 | Negative | |