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Table 4 Quantification results of exogenous DNA of the different isolation methods using DNA Fetal Kit® RHD CE (BIORAD®)

From: Qualitative and quantitative comparison of cell-free DNA and cell-free fetal DNA isolation by four (semi-)automated extraction methods: impact in two clinical applications: chimerism quantification and noninvasive prenatal diagnosis

 

MagNA Pure 24 System

IDEAL

LabTurbo 24 AB

Chemagic 360D

Ct exonegous DNA

35.61

33.65

33.08

37.22

35.61

34.37

32.52

35.64

36.24

33.79

32.59

38.39

35.42

33.42

33.25

38.66

34.66

33.32

32.86

39.38

Ct mean

35.51

33.71

32.86

37.86

SD

0.57

0.41

0.31

1.46

CV

2%

1%

1%

4%

Ct Min

34.66

33.32

32.52

35.64

Ct Max

36.24

34.37

33.25

39.38

p

< 0.005

 
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Journal of Translational Medicine

ISSN: 1479-5876

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