Fig. 5
Loss of TGFβrIII protein in breast cancer. TGFβrIII expression was analyzed by immunohistochemistry (IHC) using anti TGFβrIII (sc-74511) and chromogenic detection. IHC staining was scored on a 0 to 3 scale by a board-certified pathologist, 0 corresponding to no staining and 3 to high staining levels. H&E staining of benign breast tissue lesion (a) and breast cancer (d). Representative staining of TGFβrIII in a benign breast lesion (b, 20x; c 40x), with positive epithelial (asterisk) and myoepithelial cells (arrows). Similar results were obtained in healthy breast tissue, obtained as an internal control from each sample (g, 20x). Staining of TGFβrIII in breast cancer tissues (e, 20x; f, 40x) showed no reaction in the majority of analyzed samples. Secondary antibody only control (h); positive tissue control, benign breast tissue lesion (i). Note the significant decrease in staining intensity in breast cancer (e, f) in comparison to healthy tissues (g) and benign lesion of breast tissue (b, c)