Fig. 6

Combined treatment with MDSCs and Treg regulates human T cell and B cell response. a CD4 + T cells from healthy donor PBMC were coculture with MDSC or Treg alone or combined MDSC and Treg cells for 3 days and analyzed by flow cytometry. A plot from one representative experiment displays the proportions of IL-17 + , IFN-γ + , CD25 + Foxp3 + cells among CD4 + T cells. Numbers in the plots indicate percentages of gated cells. b Total splenocytes of normal C57BL/6 mice coculture with MDSC or Treg alone or combined MDSC and Treg cells for 3 days and analyzed by flow cytometry. A plot from one representative experiment displays the proportions of IL-10 + CD19 + cells, CD138 + B220- cells. Numbers in the plots indicate percentages of gated cells. Data are means ± SEMs. Data are representative of three independent experiments (*p < 0.05, **p < 0.01). Numbers in the plots indicate percentages of gated cells