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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: Myeloid-derived suppressor cells therapy enhance immunoregulatory properties in acute graft versus host disease with combination of regulatory T cells

Fig. 2

Combined treatment with MDSCs and Treg control alloreactive T cell response. a In the mixed lymphocyte reaction assay, a total of 1 × 105 B/c splenic T cells (responders) were incubated with 1 × 105 irradiated B/c (syngeneic stimulators, Syn) or B6 (allogeneic stimulators, Allo]) splenic APCs for 4 days. Responder cells were cultured in the presence or absence of MDSC (2 × 104) or/and Treg (2 × 104). b IFN-γ and IL-17 levels in the supernatants were measured by ELISA. c Intracellular immunostaining of IL-17 + CD4 + , IFN-γ + CD4 + , Foxp3 + Treg cells was performed were determined by flow cytometry. Data are means ± SEMs. Data are representative of three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001 versus alloresponse)

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