Fig. 2From: Myeloid-derived suppressor cells therapy enhance immunoregulatory properties in acute graft versus host disease with combination of regulatory T cellsCombined treatment with MDSCs and Treg control alloreactive T cell response. a In the mixed lymphocyte reaction assay, a total of 1âĂâ105 B/c splenic T cells (responders) were incubated with 1âĂâ105 irradiated B/c (syngeneic stimulators, Syn) or B6 (allogeneic stimulators, Allo]) splenic APCs for 4 days. Responder cells were cultured in the presence or absence of MDSC (2âĂâ104) or/and Treg (2âĂâ104). b IFN-Îł and IL-17 levels in the supernatants were measured by ELISA. c Intracellular immunostaining of IL-17â+âCD4â+â, IFN-Îłâ+âCD4â+â, Foxp3â+âTreg cells was performed were determined by flow cytometry. Data are meansâÂąâSEMs. Data are representative of three independent experiments (*pâ<â0.05, **pâ<â0.01, ***pâ<â0.001 versus alloresponse)Back to article page