Fig. 1From: Myeloid-derived suppressor cells therapy enhance immunoregulatory properties in acute graft versus host disease with combination of regulatory T cellsCombined treatment with MDSCs and Treg regulates T cell and B cell response. a CD4â+âT cell(1âĂâ106) isolated from normal C57BL/6 mice were cocultured with MDSC or Treg alone or combined MDSC(2âĂâ105) and Treg (2âĂâ105) cells for 3 days in the presence of anti-mouse CD3 antibody and analyzed by flow cytometry. A plot from one representative experiment displays the proportions of IL-17â+â, IFN-Îłâ+â, CD25â+âFoxp3â+âcells among CD4â+âT cells. Numbers in the plots indicate percentages of gated cells. b Total splenocytes (1âĂâ106) of normal C57BL/6 mice coculture with MDSC or Treg alone or combined MDSC and Treg cells for 3 days in the presence of LPS (100 ng/ml) and analyzed by flow cytometry. A plot from one representative experiment displays the proportions of IL-10â+âCD19â+âcells, CD138â+âB220- cells. Numbers in the plots indicate percentages of gated cells. Data are meansâÂąâSEMs. Data are representative of three independent experiments (*pâ<â0.05, **pâ<â0.01, ***pâ<â0.001)Back to article page