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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: Myeloid-derived suppressor cells therapy enhance immunoregulatory properties in acute graft versus host disease with combination of regulatory T cells

Fig. 1

Combined treatment with MDSCs and Treg regulates T cell and B cell response. a CD4 + T cell(1 × 106) isolated from normal C57BL/6 mice were cocultured with MDSC or Treg alone or combined MDSC(2 × 105) and Treg (2 × 105) cells for 3 days in the presence of anti-mouse CD3 antibody and analyzed by flow cytometry. A plot from one representative experiment displays the proportions of IL-17 + , IFN-γ + , CD25 + Foxp3 + cells among CD4 + T cells. Numbers in the plots indicate percentages of gated cells. b Total splenocytes (1 × 106) of normal C57BL/6 mice coculture with MDSC or Treg alone or combined MDSC and Treg cells for 3 days in the presence of LPS (100 ng/ml) and analyzed by flow cytometry. A plot from one representative experiment displays the proportions of IL-10 + CD19 + cells, CD138 + B220- cells. Numbers in the plots indicate percentages of gated cells. Data are means ± SEMs. Data are representative of three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001)

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