Fig. 2

Cancer cell-derived EVs express low levels of miR-744. a Expression of miR-744 in EVs isolated from BEAS-2B, H1299 and H522 cells detected by RT-qPCR. b Expression of miR-744 determined by RT-qPCR in A549 and H460 cells treated with BEAS-2B-, H1299-, and H522-derived EVs (* p < 0.05 vs. A549 and H460 cells treated with BEAS-2B-derived EVs). c Expression of miR-744 determined by RT-qPCR in BEAS-2B and NSCLC cells (H1299, A549, PC-9, H358, H522, and H460) (* p < 0.05 vs. BEAS-2B cells). d Expression of miR-744 in tumor and normal samples in the TCGA database. The x-axis represents the sample type and the y-axis represents the mean expression value of miR-744. e Expression of miR-744 determined by RT-qPCR in clinical samples of tumor and adjacent normal tissues (* p < 0.05 vs. normal tissues). Data are summarized as mean ± standard deviation of three technical replicates. Data from two groups were compared using unpaired t-test and data from multiple groups were assessed by one-way ANOVA with Tukey's tests