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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: Reduction of miR-744 delivered by NSCLC cell-derived extracellular vesicles upregulates SUV39H1 to promote NSCLC progression via activation of the Smad9/BMP9 axis

Fig. 1

Cancer cell-derived EVs promote NSCLC cell proliferation in vitro. a Morphology of the isolated EVs from the BEAS-2B, H1299, and H522 cells observed using TEM (scale bar: 100 nm). b EV size distribution analyzed by NAT. c EV surface markers evaluated by western blot analysis. d Representative images showing the EV internalization. After the cells were fixed, they were stained with CD63 antibody, followed by EV tracking (× 200). e Viability of untreated A549 and H460 cells and A549 and H460 cells treated with EVs isolated from BEAS-2B, H1299 and H522 cells measured by CCK-8 assay. f Colony formation of untreated A549 and H460 cells and A549 and H460 cells treated with EVs isolated from BEAS-2B, H1299 and H522 cells measured by colony formation assay. g Cell cycle distribution in untreated A549 and H460 cells and A549 and H460 cells treated with EVs isolated from BEAS-2B, H1299 and H522 cells measured by flow cytometry. h Tumor growth curve in the xenograft model mice injected with untreated A549 cells and A549 cells treated with EVs isolated from BEAS-2B, H1299 and H522 cells. i Representative images showing xenografts in nude mice injected with A549 cells treated with EVs isolated from BEAS-2B, H1299 and H522 cells. j Tumor weight of mice injected with A549 cells treated with EVs isolated from BEAS-2B, H1299 and H522 cells. k HE staining (the upper panel) showing tumor development and IHC staining (the lower panel) showing the expression of Ki-67 (× 200). Mice: n = 8/group, * p < 0.05 vs. untreated A549 and H460 cells, EVs isolated from BEAS-2B cells, or mice treated with untreated A549 cells. Data are shown as mean ± standard deviation. Data from multiple groups were assessed using one-way ANOVA with Tukey's tests. Data at difference time points were assessed by Bonferroni-corrected repeated measures ANOVA. All experiments were repeated three times independently

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