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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: Exosomal LncRNA LINC00659 transferred from cancer-associated fibroblasts promotes colorectal cancer cell progression via miR-342-3p/ANXA2 axis

Fig. 1

Characteristics of CAFs-exo and NFs-exo from patients with CRC. Phenotypes and morphologies of CAFs and NFs were observed using a laser confolcal microscope (a). Microscope graticules for measurement of CAFs and NFs (b); Western blot and immunofluorescence were used to detect the levels of α-SMA, FAP and Vimentin, bars = 100 μm (c, d). TEM was applied to detect CAFs-exo and NFs-exo, bars = 20 μm (e). The levels of CD9, CD81 and TSG101 were monitored by Western blot and and FCM (f–g). DiI-labeled exosomes were visualized via a fluorescence microscope (h). N = 3, **P < 0.01, ***P < 0.001 vs NFs group; CAFs-exo CAFs-derived exosomes, NFs-exo NFs-derived exosomes, CRC colorectal cancer, TEM transmission electron microscopy, FCM flow cytometry

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