Fig. 2

Box plots of LMWF5A-induced fold changes in nuclear transcription factor/DNA binding. Nuclear protein extracts were collected from PBMC exposed to saline or LMWF5A and stimulated with 100 ng/ml LPS or diluent control for 24 h. Transcription factor activity was then evaluated by DNA-binding ELISA, and fold changes in OD measurements were calculated for saline versus LMWF5A treatment groups for unstimulated nuclear protein samples (a), LPS-stimulated nuclear protein samples (b) and LPS-induced activation signal with unstimulated OD subtracted (c). Data are presented as fold change in OD ± IQR (n = 4–6). red line = median fold change, and * indicates p ≤ 0.05 versus hypothetical 1.0-fold change