Fig. 6

The relationship between Tespa1 and p-STAT6 detected by immunofluorescence and co-immunoprecipitation. Primary pulmonary mast cells were derived from the lungs of WT mice, and IL-4 (20 ng/mL) was simultaneously applied for 1 and 4 h. The expression and correlation of Tespa1 and p-STAT6 were detected by immunofluorescence. The relationship between Tespa1and p-STAT6 was detected by co-immunoprecipitation. a Immunofluorescence staining for Tespa1 and p-STAT6 after IL-4 stimulation. b Table of average Tespa1 and p-STAT6 fluorescence intensities. c Correlation analysis of Tespa1 and p-STAT6 expression of a. d Co-immunoprecipitation analysis of the relationship between Tespa1 and p-STAT6 in mast cells. **P < 0.01 compared with p-STAT6 at 0 min; ^##P < 0.01 compared with Tespa1 at 0 min. Mean ± SD, n = 10