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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: Tumor-associated macrophage, angiogenesis and lymphangiogenesis markers predict prognosis of non-small cell lung cancer patients

Fig. 5

Effect of macrophage polarization on NSCLC cell angiogenesis and lymphangiogenesis. A549 cells were co-cultured with M0 macrophages, M1 macrophages, polarized by LPS, or M2 macrophages, polarized by IL4 + IL13. After 48 h of co-culture, total RNA and protein were extracted from the cells as indicated. Gene expression was assessed with quantitative real-time PCR (a, b), and protein expression was quantitated using specific ELISA kits (c, d). VEGF-A and VEGF-C mRNA levels increased in M2 macrophages significantly. However, M1 macrophages elevated only VEGF-A mRNA, not VEGF-C, in A549 cells. In order to assess the levels of VEGF-A and VEGF-C mRNA in polarized macrophages, the macrophages were collected after 72 h of culturing with LPS or IL4 + IL13, total RNA and proteins were extracted, and gene and protein expression were assessed with quantitative real-time PCR (e, f) and ELISA (g, h) as well. The mRNA levels of VEGF-A and VEGF-C were significantly decreased in M2 macrophages polarized by IL4 + IL13, compared to M1 macrophages polarized by LPS or M0 macrophages cultured with vehicles. Error bars represent S.D; *p < 0.05, **p < 0.01, ***p < 0.001. Asterisk represents the significance compared to A549 alone or macrophage with vehicle

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