Fig. 2

The effect of non-synonymous mutations on 3D PDGFRα structure. Sanger sequencing chromatograms of PDGFRA exon 18 show the wild and mutated sequence of non-synonymous variations (c.2464C > T, c.2464C > A, c.2459C > T and c.2507A > T). Overviews of protein in ribbon-presentation show the protein in grey and the side chain of the mutated residue in magenta (shown as small balls). The Close-up of mutations (seen from a slightly different angle) shows the protein in grey and the side chains of both the wild-type and the mutant residue in green and red respectively. Schematic structures show the original (green) and the mutant (red) amino acid. The backbone, which is the same for each amino acid, is colored red. The side chain, unique for each amino acid, is colored black