Fig. 4

The effect of olaparib and niraparib on EMT in PARPis-sensitive AsPCs. AsPCs were grown in monolayers for 48 h and then treated with either olaparib at a concentration of 50 μM for a period of 24 h, or niraparib at a concentration of 25 μM for a period of 24 h, as non-treated AsPCs were used as controls. Western blot protein expression analysis of the two EMT markers, E-cadherin and N-cadherin in a olaparib-sensitive AsPCs and b niraparib-sensitive AsPCs. Actin was used as the loading control (n = 3). Histograms represent 3 PARPis-sensitive AsPCs, and protein expression levels were normalized to actin. The two-tailed unpaired t-test was used for statistical analysis. All values were expressed as the means ± S.D. *p < 0.05 **p < 0.01 and ***p < 0.001 c Immunofluorescence analysis of the two EMT markers E-cadherin and N-cadherin in sensitive AsPCs as compared to the no treatment control. Scale bar = 20 µm