Fig. 3

Analysis of the HR pathway activity (γH2AX foci formation) in PARPis-sensitive and resistant AsPCs. Comparative analysis of olaparib and niraparib induced formation of γ-H2AX foci. a Representative images of olaparib-induced foci formation in olaparib-sensitive AsPCs vs. b olaparib-resistant AsPCs. AsPCs were exposed to olaparib alone or to olaparib with etoposide for 48 h, the γ-H2AX foci formation was detected by immunofluorescence. c Niraparib-induced foci formation in niraparib-sensitive AsPCs vs. d niraparib-resistant AsPCs. AsPCs were exposed to niraparib alone or niraparib with etoposide for 48 h, the γ-H2AX foci formation was detected by immunofluorescence. Representative histograms are shown with DMSO used as the no treatment control (n = 3). The multiple comparison-one-way Anova-Tukey's multiple comparisons test was used for statistical analysis. Error bars denote standard deviation of each mean calculation. *p < 0.05 **p < 0.01 and ***p < 0.001. Scale bar = 10 µm