Fig. 1

Establishing ascites-derived monolayer and 3D (spheroid) primary cell cultures. a Spheroid AsPCs seeded on agarose plates 3 days and 5 days post collection. Scale bar = 50 mm. b Morphological features of spheroid AsPCs grown on tissue culture plastic plates 2 days and 6 days following seeding from agarose plates to monolayer. Scale bar = 50 mm. c Morphological features of AsPCs spheroids grown in 3D hanging drops 2 days and 4 days post seeding from agarose plates. Scale bar = 20 mm. The images are representative of (n = 25) samples