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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: Comparative analysis of adeno-associated virus serotypes for gene transfer in organotypic heart slices

Fig. 5

AAV6 cell tropism in organotypic heart slices. a To investigate AAV6-mediated GFP expression in CMs and fibroblasts, heart slices were infected with AAV6 and, on day 5, were probed with an antibody directed against vimentin and stained with DAPI, and were analyzed by confocal fluorescence microscopy. GFP-positive cells represent virus-infected cells, mCherry-positive cells indicate CMs and vimentin-positive cells indicate fibroblasts. b Enlarged CM-high area with tightly packed CMs and no apparent gaps between single cells. c Enlarged CM-low area with fewer CMs but a higher density of fibroblasts. d Percentage of GFP-positive cells per cell type. Twenty-five CMs and 25 fibroblasts with high mCherry and vimentin signals, respectively, were counted and analyzed for GFP expression. Four slices distributed over three independent experiments were quantified, and means and SDs (error bars) are given. A star indicates a significant change with p less than 0.05. n.s. indicates not significant. Scale bars, 100 µm (a) and 25 µm (b, c)

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