Fig. 4
AAV6 dose-dependent GFP expression kinetics in organotypic heart slices. a Myocardial slices were incubated with either undiluted (4 × 108 vector genomes), 1:10, or 1:100 diluted virus solutions. AAV6-mediated GFP expression was analyzed by fluorescence microscopy on day 5 after infection. Images were taken from the upper slice side. Scale bars, 100 µm. b Quantification of time-course and concentration-dependent gene transfer efficiency of AAV6. GFP-positive cells were counted on both the upper and lower slice surface on days 1, 3, and 5 after AAV infection. Three independent experiments with six slices in total were quantified, and means and SDs (error bars) are given. n.d. indicates not detected