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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Conformational changes in myeloperoxidase induced by ubiquitin and NETs containing free ISG15 from systemic lupus erythematosus patients promote a pro-inflammatory cytokine response in CD4+ T cells

Fig. 4

NETs contaning in ISG15 promote the production of IFNγ in different PBMCs subsets. IFNγ + cells from different PBMCs subsets from healthy controls were assessed by flow cytometry under different experimental conditions. A representative dot plot from one healthy control is shown for each experimental condition as follows: a Unstimulated; b NETs from healthy controls without ISG15 expression; c NETs from SLE patients that contain ISG15; d PMA + Ionomycin. Boxes (median ± interquartile range) represent the pooled data from healthy controls (n = 3) and show increased expression of IFNγ + cells when total PBMCs (e), CD4 + lymphocytes (f), CD8 + lymphocytes (g) and NK cells (h) were stimulated with SLE NETs enriched in ISG15. * p < 0.05

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