Fig. 3From: Bronchoalveolar Tregs are associated with duration of mechanical ventilation in acute respiratory distress syndromeImmune cell subsets identified in the bronchoalveolar lavage (BAL), tracheal aspirate, or peripheral blood by flow cytometry. Box-and-Whisker plots showing median, minimum, and maximum with overlay of individual data points (dots) of immune cells, expressed as a percentage of CD45+ cells at day 0 and/or day 3 after enrollment. a–d BAL immune cells expressed as a percentage of CD45+ cells at day 0 or day 3 after enrollment. a Percentages are shown for all participants at both time points. b Fast and slow responders are compared combining data from both days. c Fast and slow responders are compared at Day 0. d Fast and slow responders are compared at Day 3. e–h The tracheal aspirate immune cells expressed as a percentage of CD45+ cells at day 0 or day 3 after enrollment. Percentages are shown for participants at both time points (e), grouped as fast or slow resolvers (f), and categorized as slow or fast resolvers at Day 0 (g) or Day 3 (h). i–l The peripheral blood immune cells expressed as a percentage of CD45+ cells at day 0 or day 3 after enrollment. Percentages are shown for participants at both time points (i), grouped as fast or slow resolvers (j) and categorized as slow or fast resolvers at Day 0 (k) or Day 3 (l). The flow cytometric plots and gating scheme used for the identification of immune cells with gating were adapted from [23]. Data were analyzed per type of cell, without correction of multiple comparisons. For each cell type, repeated measure ANOVA was used to compare day 0 and 3, fast and slow resolvers, and interactionBack to article page