Fig. 9

OKN-007 increases H3.K27me3 expression, better than LDN-193189, in a DIPG model. IHC analysis staining for H3.K27me3. a The ROI’s for data analysis using IHC were placed in the heavily stained regions of the pons as circled in red. b H3.K27me3 is a marker of H3.K27M mutation post-translational modification and our results show that treatment with OKN-007 significantly increased H3.K27me3 expression compared to untreated mice or LDN-193189-treated mice. Sample legend: Untreated (UT)—closed circles (n = 4); OKN-007 (OKN)—open squares (n = 4); and LDN-193189 (LDN)—closed triangles (n-5). An ANOVA (one-way ANOVA, multiple comparisons) statistical test was used. *p < 0.05, **p < 0.01 and ***p < 0.001. c The 20× view of IHC slides visibly show the lowest H3.K27me3 expression in the untreated mice compared to the d OKN-007 treated mice. There was no significant difference between e LDN-193189 treated mice and untreated mice. The scale bar in each image frame is 100 µm