Fig. 8

OKN-007 and LDN-193189 both decrease ACVR1 in a DIPG model. IHC analysis staining for ACVR1 antibody. ACVR1 is a common mutation found in DIPG, second to H3 K27M mutation. a The ROI’s for data analysis of IHC were placed in the heavily stained regions of the pons as circled in red. b Cell stain positivity for ACVR1 antibody was higher in the untreated mice compared to both treatment groups. There was no difference between the two treatment arms. Sample legend: Untreated (UT)—closed circles (n = 4); OKN-007 (OKN)—open squares (n = 4); and LDN-193189 (LDN)—closed triangles (n-5). An ANOVA (one-way ANOVA, multiple comparisons) statistical test was used. ****p < 0.0001, and ns is not significant. c The 20× view of IHC slides visibly show higher cell stain positivity for ACVR1 in the untreated mice compared to the d OKN-007 treated and e LDN-193189 treated mice. The scale bar in each image frame is 100 µm