Fig. 12

OKN-007 and LDN-193189 both decrease c-MET in a DIPG model. IHC analysis staining for c-MET. c-MET is highly expressed in half of DIPG cases. a The ROI’s for data analysis of IHC were placed in the heavily stained regions of the pons as circled in red. b Data suggests that untreated mice had significantly higher c-MET expression compared to both treatment groups. There was no significant difference in c-MET expression between the OKN-007 and LDN-193189 treated mice. Sample legend: Untreated (UT)—closed circles (n = 4); OKN-007 (OKN)—open squares (n = 4); and LDN-193189 (LDN)—closed triangles (n-5). An ANOVA (one-way ANOVA, multiple comparisons) statistical test was used. *p < 0.05, **p < 0.01, and ns is not significant. c The 20× view of IHC slides visibly show higher cell stain positivity for c-MET in the untreated mice compared to the d OKN-007 treated and e LDN-193189 treated mice. The scale bar in each image frame is 100 µm